鸡基质蛋白3核定位信号的预测与鉴定

doi:10.11843/j.issn.0366-6964.2018.11.021

Abstract

Abstract:

This study aimed to predict and identify the nuclear localization signal (NLS) of chicken matrin 3 (MATR3). The putative NLS (pNLS) in chicken MATR3 was predicted by the online NLS prediction software. The recombinant plasmids of chicken MATR3 with the pNLS deletion were constructed and then transfected into cells. The subcellular localization of the pNLS deletants was observed and analyzed to determine the NLS of chicken MATR3. The recombinant plasmids expressing the deletion of basic amino acids close to NLS were also constructed to examine their roles in the nuclear localization of chicken MATR3. In addition, the conservation analysis and nuclear import ability of chicken MATR3 NLS were investigated. The results showed that there were four pNLS existed in chicken MATR3, and the deletion of pNLS2 ⁽⁵⁹⁶PSDKKSK⁶⁰²) disrupted the nuclear localization of chicken MATR3. In addition, the constructed recombinant plasmids expressing the deletion of basic amino acids close to NLS were transfected into cells, and the subcellular localization of the recombinant proteins indicated that the adjacent basic amino acids of NLS did not participate in the NLS-mediated nuclear localization of chicken MATR3. Moreover, we also found that the NLS motif in MATR3 protein were conserved among different species, and also had the same nuclear import ability as the NLS of SV40 large T antigen. One highly conservative NLS (⁵⁹⁶PSDKSK⁶⁰²) is present in chicken MATR3, and its adjacent basic amino acids do not participate in the nuclear localization of MATR3 protein.

CLC Number:

S831
Q71

DENG Shan-shan, GAO Hong-bo, YUAN Chao, ZHAO Jia-fu, NI Meng-meng, DUAN Zhi-qiang, JI Xin-qin. Prediction and Identification of Nuclear Localization Signal of Chicken Matrin 3[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(11): 2486-2495.

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References

[1] NALAYASU H, BEREZNEY R. Nuclear matrins:Identification of the major nuclear matrix proteins[J]. PNAS, 1991, 88(22):10312-10316.
[2] BELGRADER P, DEY R, BEREZNEY R. Molecular cloning of matrin 3.
A 125-kilodalton protein of the nuclear matrix contains an extensive acidic domain[J]. J Biol Chem, 1991, 266(15):9893-9899.
[3] HIBINO Y, USUI T, MORITA Y, et al. Molecular properties and intracellular localization of rat liver nuclear scaffold protein P130[J]. Biochim Biophys Acta, 2006, 1759(5):195-207.
[4] COELHO M B, ATTIG J, BELLORA N, et al. Nuclear matrix protein matrin3 regulates alternative splicing and forms overlapping regulatory networks with PTB[J]. EMBO J, 2015, 34(5):653-668.
[5] BEAUSOLEIL S A, JEDRYCHOWSKI M, SCHWARTZ D, et al. Large-scale characterization of HeLa cell nuclear phosphoproteins[J]. Proc Natl Acad Sci U S A, 2004, 101(33):12130-12135.
[6] COELHO M B, ATTIG J, ULE J, et al. Matrin3:Connecting gene expression with the nuclear matrix[J]. Wiley Interdiscip Rev RNA, 2016, 7(3):303-315.
[7] KULA A, GUERRA J, KNEZEVICH A, et al. Characterization of the HIV-1 RNA associated proteome identifies Matrin 3 as a nuclear cofactor of Rev function[J]. Retrovirology, 2011, 8:60.
[8] YEDAVALLI V S, JEANG K T. Matrin 3 is a co-factor for HIV-1 Rev in regulating post-transcriptional viral gene expression[J]. Retrovirology, 2011, 8:61.
[9] SOROKIN A V, KIN E R, OVCHINNIKOV L P. Nucleocytoplasmic transport of proteins[J]. Biochemistry (Moscow), 2007, 72(13):1439-1457.
[10] WOERNER A C, FROTTIN F, HORNBURG D, et al. Cytoplasmic protein aggregates interfere with nucleocytoplasmic transport of protein and RNA[J]. Science, 2016, 351(6269):173-176.
[11] XU D, FARMER A, CHOOK Y M. Recognition of nuclear targeting signals by karyopherin-β proteins[J]. Curr Opin Struct Biol, 2010, 20(6):782-790.
[12] 邓珊珊,嵇辛勤,段志强,等. 鸡基质蛋白3基因的生物信息学分析[J]. 中国家禽, 2017, 39(19):67-70.
DENG S S, JI X Q, DUAN Z Q, et al. Bioinformatics analysis of chicken matrix protein 3 gene[J]. Chinese Poultry, 2017, 39(19):67-70. (in Chinese)
[13] COHEN T V, HERNANDEZ L, STEWART C L. Functions of the nuclear envelope and lamina in development and disease[J]. Biochem Soc Trans, 2008, 36(6):1329-1334.
[14] SALTON M, ELKON R, BORODINA T, et al. Matrin 3 binds and stabilizes mRNA[J]. PLoS One, 2011, 6(8):e23882.
[15] BERNERT G, FOUNTOULAKIS M, LUBEC G. Manifold decreased protein levels of matrin 3, reduced motor protein HMP and hlark in fetal Down's syndrome brain[J]. Proteomics, 2002, 2(12):1752-1757.
[16] MOLONEY C, RAYAPROLU S, HOWARD J, et al. Transgenic mice overexpressing the ALS-linked protein matrin 3 develop a profound muscle phenotype[J]. Acta Neuropathol Commun, 2016, 4(1):122.
[17] MARANGI G, LATTANTE S, DORONZIO P N, et al. Matrin 3 variants are frequent in Italian ALS patients[J]. Neurobiol Aging, 2017, 49:218.
e1-218.
e7.
[18] DUAN Z Q, XU H X, JI X Q, et al. Importin α5 negatively regulates importin β1-mediated nuclear import of Newcastle disease virus matrix protein and viral replication and pathogenicity in chicken fibroblasts[J]. Virulence, 2018, 9(1):783-803.
[19] 段志强,胡顺林,刘秀梵. 新城疫病毒与其它副黏病毒基质蛋白功能的比较[J]. 微生物学报, 2016, 56(7):1070-1078.
DUAN Z Q, HU S L, LIU X F. Function comparison of the matrix protein between Newcastle disease virus and other paramyxoviruses-A review[J]. Acta Microbiologica Sinica, 2016, 56(7):1070-1078. (in Chinese)
[20] WILLIS A N, DEAN S E, HABBOUCHE J A, et al. Nuclear localization signal sequence is required for VACM-1/CUL5-dependent regulation of cellular growth[J]. Cell Tissue Res, 2017, 368(1):105-114.
[21] CHRISTOPHE D, CHRISTOPHE-HOBERTUS C, PICHON B. Nuclear targeting of proteins:How many different signals?[J]. Cell Signal, 2000, 12(5):337-341.
[22] SHIBATA A, MACHIDA J, YAMAGUCHI S, et al. Identification of nuclear localization signals in the human homeoprotein MSX1[J]. Biochem Cell Biol, 2018, 96(4):483-489.
[23] DINGWALL C, LASKEY R A. Nuclear targeting sequences-a consensus?[J]. Trends Biochem Sci, 1991, 16(12):478-481.
[24] CONTI E, MÖLLER C W, STEWART M. Karyopherin flexibility in nucleocytoplasmic transport[J]. Curr Opin Struct Biol, 2006, 16(2):237-244.
[25] KIMURA M, IMAMOTO N. Biological significance of the importin-β family-dependent nucleocytoplasmic transport pathways[J]. Traffic, 2014, 15(7):727-748.
[26] SUN M, LONG J, YI Y X, et al. Importin α-importin β complex mediated nuclear translocation of insulin-like growth factor binding protein-5[J]. Endocr J, 2017, 64(10):963-975.
[27] ROWE C L, WAGSTAFF K M, OKSAYAN S, et al. Nuclear trafficking of the rabies virus interferon antagonist P-protein is regulated by an importin-binding nuclear localization sequence in the C-terminal domain[J]. PLoS One, 2016, 11(3):e0150477.
[28] PUMROY R A, KE S, HART D J, et al. Molecular determinants for nuclear import of influenza A PB2 by importin α isoforms 3 and 7[J]. Structure, 2015, 23(2):374-384.

Prediction and Identification of Nuclear Localization Signal of Chicken Matrin 3

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